Determining biological noise via single cell analysis
نویسندگان
چکیده
منابع مشابه
Single-cell analysis uncovers extensive biological noise in poliovirus replication.
UNLABELLED Viral infections often begin with a very small number of initiating particles. Accordingly, the outcome of an infection is likely to be affected by variability in the initial molecular interactions between virus and host. In this study, we investigated the range of outcomes upon infection of single cells. We isolated individual cells infected with poliovirus at low or high multiplici...
متن کاملSingle-molecule identification via electric current noise
Label-free and real-time single-molecule detection may aid the development of high-throughput biosensing platforms. Molecular fluctuations are a source of noise that often hinders single-molecule identification by obscuring the fine details of molecular identity. In this study, we report molecular identification through direct observation of quantum-fluctuation-induced inelastic noise in single...
متن کاملChallenging biological limits with microfluidic single cell analysis
Back in the 17th century, van Leeuwenhoek was the first to advance into the microbial universe with his simple, but powerful single lens microscope. In a drop of aqueous infusion, he saw for the first time the basic functional and replicating units of life: single cells, in the shape of individual microbes. This observation laid the foundation for modern (micro-) biology. What began with a sing...
متن کاملUnravelling the biological secrets of microchimerism by single-cell analysis.
The presence of microchimeric cells is known for >100 years and well documented since decades. Earlier, microchimeric cells were mainly used for cell-based non-invasive prenatal diagnostics during early pregnancy. Microchimeric cells are also present beyond delivery and are associated to various autoimmune diseases, tissue repair, cancer and immune tolerance. All these findings were based on lo...
متن کاملQuantification noise in single cell experiments
In quantitative single-cell studies, the critical part is the low amount of nucleic acids present and the resulting experimental variations. In addition biological data obtained from heterogeneous tissue are not reflecting the expression behaviour of every single-cell. These variations can be derived from natural biological variance or can be introduced externally. Both have negative effects on...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2008
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-008-2431-z